Mixing Volume control

Enter a number in the text box to indicate the amount of "mixing volume" (in uL) present before the column inlet.

Remarks

Usually, the majority of the mixing volume is in the pump itself and in the solvent mixer. This mixing volume is depicted in the diagram below:

Consider a gradient in which the fraction of solvent B increases linearly over the course of the experiment. As eluent with a higher amount of solvent B enters the mixing volume, it mixes with eluent of a lower solvent B fraction. This causes the gradient profile reaching the column to be dispersed and delayed.

The graph below shows an ideal gradient profile going from 0% B to 100% B in 5 min without any mixing volume.

The graph below shows the same gradient profile distored from a large mixing volume.